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Multiple Choice

Sabouraud agar is most appropriate for isolating which group?

Sabouraud dextrose agar is designed to favor fungal growth while suppressing bacteria, making it the go-to medium for isolating fungi from clinical specimens. The medium uses a relatively high sugar content and a low pH around 5.6, which many bacteria find inhibitory, whereas fungi—such as yeasts and moulds, including dermatophytes—thrives under these conditions. Sometimes antibiotics are added to further reduce bacterial contamination, and additives like cycloheximide can help differentiate certain fungi. This is not suitable for bacteria, which prefer neutral or higher pH environments and different nutrient profiles; viruses require living host cells for propagation and aren’t cultured on standard agar; parasites typically need specialized media tailored to their growth.

Sabouraud dextrose agar is designed to favor fungal growth while suppressing bacteria, making it the go-to medium for isolating fungi from clinical specimens. The medium uses a relatively high sugar content and a low pH around 5.6, which many bacteria find inhibitory, whereas fungi—such as yeasts and moulds, including dermatophytes—thrives under these conditions. Sometimes antibiotics are added to further reduce bacterial contamination, and additives like cycloheximide can help differentiate certain fungi. This is not suitable for bacteria, which prefer neutral or higher pH environments and different nutrient profiles; viruses require living host cells for propagation and aren’t cultured on standard agar; parasites typically need specialized media tailored to their growth.